First record of Bursaphelenchus rainulfi on pine trees from eastern China and its phylogenetic relationship with intro-genus species.
- Author:
Li-qin JIANG
1
;
Xu-qing LI
;
Jing-wu ZHENG
Author Information
1. Institute of Biotechnology, School of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Biological Evolution;
China;
DNA, Helminth;
genetics;
Nematoda;
anatomy & histology;
genetics;
Phylogeny;
Pinus;
parasitology;
Species Specificity
- From:
Journal of Zhejiang University. Science. B
2007;8(5):345-351
- CountryChina
- Language:English
-
Abstract:
Bursaphelenchus rainulfi isolated from dead pine trees in Zhejiang, China, is described and illustrated. It also provided some molecular characters of the Chinese population, including the PCR-RFLP and sequences of ITS region and D2-D3 expansion region of the large subunit (LSU) rRNA gene. Both the morphological characters and ITS-RFLP patterns match with the original description. The phylogenetic trees based on the 13 sequences of D2-D3 expansion region of the LSU rRNA gene and ITS region of Bursaphelenchus species were constructed, respectively, with the results showing the similar clades. The phylogenetic relationship based on the molecular data is similar to that with morphological characters. This is the first report of the species on pine wood in eastern China.