Effect of bilirubin on expression of toll-like receptor 4 in cord blood monocytes.
- Author:
Hui-Ling LIU
1
;
Chang-Hui CHEN
;
Min CHEN
;
Qing WU
;
Mao-Jun LI
Author Information
- Publication Type:Journal Article
- MeSH: Bilirubin; pharmacology; Dose-Response Relationship, Drug; Fetal Blood; chemistry; drug effects; Humans; Infant, Newborn; Monocytes; chemistry; drug effects; Toll-Like Receptor 4; blood
- From: Chinese Journal of Contemporary Pediatrics 2011;13(2):153-156
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of different concentrations of bilirubin on expression of toll-like receptor 4 (TLR4) in cord blood monocytes (CBMC).
METHODSUnder the sterile condition, umbilical vein blood samples were obtained from normal full-term newborns, and the monocytes were in vitro separated by the method of gelatin/plasma coated flasks. The monocytes were preincubated with various concentrations (0-307.8 μmol/L) of bilirubin dissolved in bovine albumin solution for 1 hr. Bilirubin-treated CBMC were further cultured with LPS (1 μg/mL) to induce cellular activation for 24 hrs, and then the CBMC were collected. The expression of TLR4 in monocytes was measured by indirect immunofluorescence method.
RESULTSBilirubin at the concentrations of 102.6, 153.9, 220.6 and 307.8 μmol/L inhibited the expression of TLR4 of CBMC. The inhibition effect increased with the increasing concentration of bilirubin.
CONCLUSIONSBilirubin can inhibit the TLR4 expression of CBNC in a dose-dependent manner.
