Value of fluorescence quantitative PCR assay of bronchoalveolar lavage fluid samples in the diagnosis of Mycoplasma pneumoniae pneumonia in children.
- Author:
Li-Li ZHONG
1
;
Li PENG
;
Han HUANG
;
Mo LIANG
;
Qiong-Hua ZHOU
;
Bing ZHANG
;
Yun LI
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Bronchoalveolar Lavage Fluid; microbiology; Child; Child, Preschool; DNA, Bacterial; analysis; Female; Fluorescence; Gene Dosage; Humans; Infant; Infant, Newborn; Male; Pneumonia, Mycoplasma; diagnosis; Polymerase Chain Reaction; methods; Sensitivity and Specificity
- From: Chinese Journal of Contemporary Pediatrics 2011;13(3):191-194
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the value of fluorescence quantitative polymerase chain reaction (FQ-PCR) for detecting Mycoplasma pneumoniae (MP)-DNA in bronchoalveolar lavage fluid (BALF) in the early diagnosis of Mycoplasma pneumoniae pneumonia (MPP).
METHODSFQ-PCR was used to detect MP-DNA in BALF obtained by fiberoptic bronchoscopy from 61 children with MPP, and the sensitivity and the specificity of FQ-PCR were compared with the traditional serological test.
RESULTSThe sensitivity and the specificity of BALF FQ-PCR for detecting MP-DNA were 94% and 100% respectively. The accuracy of BALF FQ-PCR assay for detecting MP-DNA was higher than that of the serological test at the early stage of the disease (1-7 days) (P<0.01). In the children with refractory MPP, BALF FQ-PCR assay also showed higher accuracy for detecting MP-DNA than the serological test (P<0.01). The copies of MP-DNA in children with refractory MPP were significant higher than those in children with common MPP (P<0.05). The copies of MP-DNA were positively correlated with CRP values (r=0.845, P<0.01).
CONCLUSIONSFQ-PCR assay of BALF for detecting MP-DNA in BALF is superior to the serological test. It is a reliable method for the early diagnosis of MPP, especially refractory MPP. The copies of MP-DNA can be used as an index for evaluation of the treatment outcome of refractory MPP.
