Detection and significance of CD4+ CD25int/high CD127low regulatory T cells in pediatric aplastic anemia.
- Author:
Xi-Ge WANG
1
;
Yi-Ming CAO
;
Xiao-Ge WANG
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Anemia, Aplastic; immunology; Child; Child, Preschool; Female; Humans; Infant; Interleukin-7 Receptor alpha Subunit; analysis; Male; T-Lymphocytes, Regulatory; immunology
- From: Chinese Journal of Contemporary Pediatrics 2011;13(4):292-295
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the expression of CD4+ CD25int/high CD127low regulatory T cells in peripheral blood (PB) and its relation to the quantity of Hb, WBC and platelet (Plt) in children with aplastic anemia (AA).
METHODSExpression of CD4+ CD25int/high CD127low in PB was detected by flow cytometry in 22 children with AA before and after treatment and in 15 healthy controls. The relationships between CD4+CD25highCD127low and the quantity of Hb, WBC and Plt were evaluated.
RESULTSCompared to controls, the percentages of CD4+ CD25+/CD4+, CD4+CD25high/CD4+, CD4+ CD25+ CD127low/CD4+ and CD4+CD25highCD127low/CD4+ in PB of AA patients decreased markedly at the active phase (P﹤0.05). By the recovery phase, the percentages of CD4+CD25+/CD4+, CD4+CD25high/CD4+, CD4+ CD25+ CD127low/CD4+ and CD4+CD25highCD127low/CD4+ increased significantly to the levels similar to the controls. There were significant positive relationships between the expression of CD4+CD25highCD127low cells and the quantity of Hb, WBC and Plt (r=0.499, 0.526, 0.540 respectively; P﹤0.05).
CONCLUSIONSThe decrease of the percentage of CD4+CD25int/highCD127low regulatory T cells might be associated with the development of pediatric AA. The CD4+CD25int/highCD127low regulatory T cells can serve as a marker for the evaluation of disease severity as well as a target of further study on immune treatment of AA.
