HPLC fingerprint of Liuwei Dihuang condensed pills.
- Author:
Xin-Biao GAO
1
;
Lei SUN
;
Shan-Yi QIAO
;
Song GAO
;
Yan-Zhong CHE
;
Ke-Rong ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; methods; Drugs, Chinese Herbal; analysis; standards; Quality Control; Tablets; analysis
- From: China Journal of Chinese Materia Medica 2012;37(22):3411-3415
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish HPLC fingerprints of Liuwei Dihuang condensed pills.
METHODDikma Diamonsil C18 column (4.6 mm x 250 mm, 5 microm) was adopted, with acetonitrile (containing 0.05% phosphoric) -water (containing 0.05% phosphoric) as the mobile phase. The column temperature was set at 40 degrees C, and the flow rate was 1.0 mL x min(-1). The detection wavelength was 276 nm (0-10 min), 236 nm (10-40 min) and 276 nm (40-60 min). The sample size was 20 microL. Chromatographic peaks were identified by Q-TOF-MS-IDA-MS/MS method.
RESULTGood precision, stability and repeatability were proved. Q-TOF-MS-IDA-MS/ MS method was adopted for qualitative determination of eighteen chromatographic peaks. Ten batches of Liuwei Dihuang condensed pills were determined with the method, and their similarities were above 0. 96.
CONCLUSIONThe study lays a foundation for the overall quality evaluation of Liuwei Dihuang condensed pills.
