Effect of ginsenoside Rg1 on functional expression of human neural stem cells: a patch clamp study.
- Author:
Ying-Hong JIANG
1
;
Ying-Bo LI
;
Xiang-Qin ZHAO
;
Di CHEN
;
Rong JIANG
;
Sha-Li WANG
Author Information
- Publication Type:Journal Article
- MeSH: Cells, Cultured; Gene Expression; drug effects; Ginsenosides; pharmacology; Humans; Membrane Potentials; drug effects; Neural Stem Cells; cytology; drug effects; Patch-Clamp Techniques; Plant Extracts; pharmacology; Potassium Channels; genetics; metabolism; Sodium Channels; genetics; metabolism
- From: China Journal of Chinese Materia Medica 2012;37(22):3477-3480
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the effects of ginsenoside Rg1 on the functional expression of human neural stem cells (hNSCs).
METHODThe membrane electrophysiological properties and sodium and potassium ion channels in the hNSCs induced by Rg1 were analyzed using the whole-cell patch-clamp.
RESULTOn the 7th day, the neuron-like cells derived from ginsenoside Rg1 (20 mg x L(-1))-induced NSCs show: (1) The resting membrane potential: (-45.70 +/- 2.63) mV, the membrane capacitance: (26.89 +/- 1.91) pF, the membrane input impedance: (877.51 +/- 20.44) MH (P < 0.05 compared with the control group, respectively); (2) The detection rate of inward sodium current which is rapidly activated and inactivated in voltage-dependence was 50%, and its average peak value was (711.48 +/- 158.03) pA (P < 0.05 compared with the control group); (3) The outward potassium currents were composed of rapidly activated and inactivated transient outward potassium current and delayed rectifier outward potassium current, and its average peak value was (1 070.42 +/- 177.18) pA (P < 0.05 compared with the control group).
CONCLUSIONGinsenoside Rg1 can promote the functional expression and maturity of hNSCs.
