Protective effect of Qihuang Mingmu capsule on retina of diabetic mice and its impact on VEGF expression.
- Author:
Hong-Tao WANG
1
;
Chao WANG
;
Hui-Ming ZHU
;
Gang WEI
;
Shao-Hua ZHAO
;
Xiao-Lin QI
;
Hui-Xin ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Capsules; administration & dosage; Diabetic Retinopathy; drug therapy; genetics; metabolism; prevention & control; Drugs, Chinese Herbal; administration & dosage; Gene Expression; drug effects; Humans; Male; Mice; Mice, Inbred C57BL; Protective Agents; administration & dosage; Retinal Diseases; drug therapy; genetics; metabolism; prevention & control; Vascular Endothelial Growth Factor A; genetics; metabolism
- From: China Journal of Chinese Materia Medica 2012;37(22):3481-3485
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the protective effect of Qihuang Mingmu capsule (QHMM) on retina of diabetic mice and its impact on VEGF expression.
METHODForty KK/Upj-Ay mice were randomly divided into the model group and high, middle and low dose QHMM (8.32, 4.16, 2.08 g x kg(-1)) groups. Additional 10 C57BL/6 mice were selected as the control group. Mice were orally administered for three months. Their general appearance, fasting blood-glucose (FBG) and glycosylated hemoglobin (HbA1c) were observed. Pathological changes of retina were observed by light microscope and electron microscope. The expressions of vascular endothelial growth factor (VEGF), growth factor receptors-1 (Flt-1) and growth factor receptors-2 (Flk-1) were examined by Real-time PCR (qPCR) and Western blot.
RESULTQHMM could ameliorate the symptoms of diabetic mice to varying degrees, decrease FBG and HbA1c, alleviate pathological lesions of retina and decrease the expressions of VEGF, Flt-1, Flk-1 mRNA and protein.
CONCLUSIONQHMM has the protective effect on diabetic retinopathy of mice by inhibiting the expressions of VEGF, Flt-1 and Flk-1 and intervening VEGF-VEGFR signal transduction pathway.
