Chemical constituents contained in fatty oil from seeds of Cucumis sativus.
- Author:
Xiao-Yi WU
1
;
Zhi-Mao CHAO
;
Chun WANG
;
Zhi-Gao TAN
;
Wen SUN
Author Information
- Publication Type:Journal Article
- MeSH: Cucumis sativus; chemistry; Fatty Acids; analysis; Plant Oils; analysis; Seeds; chemistry
- From: China Journal of Chinese Materia Medica 2012;37(21):3252-3255
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the chemical constituents contained in the seeds of Cucumis sativus.
METHODThe fatty oil was extracted by heating and refluxing with petroleum ether. Potassium hydroxide-methanol solution was used for saponification. An unsaponifiable matter was extracted by EtOAc and separated with various chromatographic methods. Its structure was identified on the basis of their physicochemical properties and spectral data. The fatty acid fraction was methyl-esterified and determined by GC. The composition and relative content of fatty acid were determined with normalization method of peak area.
RESULT24-ethylcholesta-7, 22, 25-trienol (1), 24-ethylcholesta-7, 25-dienol (2) ,avenasterol (3), spinasterol (4), karounidiol (5) and isokarounidiol (6) were separated and identified from the unsaponifiable matter. Myristic acid (7, 0.12%), palmitic acid (8, 12.04%), palmitoleic acid (9, 0.09%), heptadecanoic acid (10, 0.06%), stearic acid (11, 5.64%), oleic acid (12, 6.95%), linoleic acid (13, 74.40%), arachidic acid (14, 0.19%), and alpha-linolenic acid (15, 0.51%) were identified from the fatty acids part.
CONCLUSIONCompounds 5, 6, 9, 10, 14,and 15 were first reported in C. sativus.
