Experimental study on effect of different chromatographic conditions on QAMS relative correlation factors and relative retention values.
- Author:
Wei-Hong FENG
1
;
Fei YANG
;
Zhi-Min WANG
;
Qi-Wei ZHANG
;
Dong-Ying LI
;
Li-Na JI
;
Yu-Ying WANG
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; methods; Rheum; chemistry
- From: China Journal of Chinese Materia Medica 2012;37(21):3264-3267
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of different chromatographic conditions on QAMS relative correlation factors (RCF) and relative retention values (RT(R)).
METHODC18 columns were used with methyl alcohol-0.4% phosphoric acid water (85: 15) as the mobile phase. The detection wavelength was 254 nm, the column temperature was 30 degrees C, and the flow rate was 1.0 mL x min(-1). The five anthraquinones in Rhei Radix et Rhizoma were selected to be the objects of study. The RCF and RT(R) among aloe-emodin, rhein, chrysophanol, physcion and emodin were determined under different chromatographic conditions.
RESULTTheir RCFs showed no significant difference.
CONCLUSIONThe RCFs among anthraquinones established by QAMS can be used as a constant in content determination of traditional Chinese medicines/patent traditional Chinese medicines.
