Content determination of two isomers containd in Garcinia hanburyi by HPLC.
- Author:
Jun-Yan ZHANG
1
;
Guang-Ping XIA
;
Na-Xia ZHAO
;
Yun-Ping CHANG
;
Ying-Mei HAN
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; methods; Garcinia; chemistry; Isomerism; Linear Models; Xanthones; analysis
- From: China Journal of Chinese Materia Medica 2012;37(21):3268-3270
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a method for determing the content of two isomers containd in Garcinia hanburyi by HPLC.
METHODChromatographic column of SunFire (Waters) C8 (2.1 mm x 150 mm, 3.5 microm) was adopted, with acetonitrile-methanol-0.3% trifluoroacetic acid (36: 37:27) as the mobile phase. The detection wavelength was 360 nm,the flow rate was 0.3 mL x min(-1), and the column temperature was 28 degrees C.
RESULTThe linear regression equation of r-gambogic acid was Y = 2.87 x 10(6) X - 2.24 x 10(5), r = 0.999 9. The linear regression equation of S-gambogic acid was Y = 3.31 x 10(6) X - 1.44 x 10(5), r = 0.999 9. The average recoveries were 100.0% and 100.9%, with RSD being 2.1% and 2.5% (n = 6), respectivley. The average contents of two gambogic acid in G. hanburyi were 30.06% and 21.45%, respectively.
CONCLUSIONThe method was so convenient and stable that it can be used for identification and content determination of two isomers containd in G. hanburyi.
