Protective effect of qingxin kaiqiao fang containing cerebrospinal fluid on PC12 cell injury induced by Abeta25-35.
- Author:
Guang-Hua YE
1
;
Hai-Yan HU
;
Xiang CHEN
;
Wen-Hua WANG
;
Xiao-Yan ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Amyloid beta-Peptides; toxicity; Animals; Apoptosis; drug effects; Caspase 3; genetics; Cell Survival; drug effects; Cerebrospinal Fluid; Male; Medicine, Chinese Traditional; Neuroprotective Agents; pharmacology; PC12 Cells; Peptide Fragments; toxicity; Proto-Oncogene Proteins c-bcl-2; genetics; Rats; Rats, Sprague-Dawley; bcl-2-Associated X Protein; genetics
- From: China Journal of Chinese Materia Medica 2012;37(21):3271-3274
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the protective effect of Qingxin Kaiqiao Fang containing cerebrospinal fluid on PC12 cell injury induced by Abeta25-35, in order to provide basis for clinical application of the formula.
METHODSprague Dawley rats were orally administration with Qingxin Kaiqiao Fang (7.9 g x kg(-1)) twice a day for 3.5 days to prepare Qingxin Kaiqiao Fang containing cerebrospinal fluid. The nerve cell injury model was established by PC12 cells and Abeta25-35 with the concentration of 10 micromol x L(-1). The expressions of Bax, Bcl-2 and Caspase-3 mRNA were detected by immunohistochemical method in the PC12 cells.
RESULTThe Qingxin Kaiqiao Fang group showed a significant higher PC12 cell activity than the model group, with decrease in Bax mRNA and Caspase-3 mRNA expressions and increase in Bcl-2 mRNA expression. There was a significant difference from the model group (P < 0.05, P < 0.01).
CONCLUSIONQinxin Kaiqiao Fang shows a significant protective effect on Abeta25-35-induced nerve cell injury.
