Study on effect of total coumarins from Urtica dentata on dextran sulfate sodium-induced colitis in mice.
- Author:
Jing-Li LU
1
;
Wei-Jie LI
;
Wen-Rui HOU
;
Yue LAN
;
Hong ZHOU
;
Li-Jiao YIN
;
Ying ZENG
;
Ming XIANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Colitis; drug therapy; metabolism; Coumarins; therapeutic use; Cytokines; blood; Dextran Sulfate; toxicity; Disease Models, Animal; Male; Mice; Mice, Inbred BALB C; NF-kappa B; antagonists & inhibitors; Toll-Like Receptor 4; antagonists & inhibitors; Urticaceae; chemistry
- From: China Journal of Chinese Materia Medica 2012;37(21):3316-3320
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of total coumarins (TC) from Urtica dentata on dextran sulfate sodium (DSS)-induced colitis in mice.
METHODThe colitis model was established by administering DSS. Having been treated with TC, their body weight was determined. Concentrations of IL-6, IL-10, TGF-beta1 and IFN-gamma were monitored by ELISA. Colon samples were collected for the histopathological examination. Western blot was used to detect TLR4 and NF-kappaB protein expression in colonic tissues.
RESULTTCs from U. dentata effectively controlled the body weight loss of mice with colitis, down-regulated the concentration of IL-6 and IFN-gamma and increased the suppressive cytokines IL-10 and TGF-beta1 in the serum. Additionally, TC alleviated the mucosal damage and decreased the expressions of TLR4 and NF-kappaB in colonic tissues.
CONCLUSIONTCs from U. dentata shows the anti-inflammatory effect on colitis in mice by reducing the expressions of TLR4 and NF-kappaB in colonic tissues and regulating pro-and anti-inflammatory cytokines.
