Effects of acitretin on the expression of signaling pathway-related genes in epidermal squamous-cell carcinoma cells.

Sui-qing Cai; Li-rong Chen; Min Zheng

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Effects of acitretin on the expression of signaling pathway-related genes in epidermal squamous-cell carcinoma cells.

Author: Sui-qing CAI ¹ ; Li-rong CHEN ; Min ZHENG
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1. Department of Dermatology, Second Affiliated Hospital of Zhejiang University, School of Medicine, Hangzhou 310009, China.
Publication Type:Journal Article
MeSH: Acitretin; pharmacology; Antineoplastic Agents; pharmacology; Carcinoma, Squamous Cell; metabolism; pathology; Cell Line, Tumor; Cyclin D1; biosynthesis; genetics; Down-Regulation; Gene Expression Regulation, Neoplastic; Humans; Mitogen-Activated Protein Kinase 1; biosynthesis; genetics; Mitogen-Activated Protein Kinase 3; biosynthesis; genetics; RNA, Messenger; biosynthesis; genetics; STAT3 Transcription Factor; biosynthesis; genetics; Signal Transduction
From: Chinese Journal of Oncology 2006;28(1):21-24
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo study the effects of acitretin on the expression of signaling pathway-related genes in an epidermal squamous-cell carcinoma cell line.
METHODSThe mRNA expression levels of STAT3, cyclin D1 and p42/44MAPK were detected in a human epidermal carcinoma cell line A431 by RT-PCR. Their expressions at protein level were studied by Western blot. The expression levels were studied in cells treated with or without 10(-5) mol/L acitretin at different time intervals.
RESULTS(1) Acitretin could significantly inhibit the expression of STAT3 and cyclin D1 mRNA in a time-dependent manner (P < 0.05). The STAT3 and cyclin D1 protein expression levels were down-regulated. Acitretin could also down-regulate the p42/4MAPK mRNA expression. (2) After incubation with acitretin, the mRNA level of cyclin D1 cells was positively correlated with that of STAT3 (P < 0.05). The cyclin D1 protein level was also positively correlated with that of STAT3 (P < 0.05). However there was no correlation of mRNA levels between cyclin D1 and p42/44MAPK.
CONCLUSIONRegulation of the Jak/STAT3 signaling pathway may play an important role in the effect of acitretin on epidermal squamous-cell carcinoma cells. The abnormal expression of STAT3 can be regarded as a prerequisite for acitretin treatment effect.