Immunoglobulin expression in colon cancer cell line HT-29 and its biological activities.
- Author:
Yu-qing DENG
1
;
Jie ZHENG
;
Guo-hui LI
;
Xiao-hui ZHU
;
Pei ZHANG
;
Jing HUANG
;
Ying-mei ZHANG
;
Zhi-xin LI
;
Xiao-yan QIU
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; Cell Proliferation; Complementarity Determining Regions; biosynthesis; genetics; DNA, Antisense; genetics; Electroporation; Genetic Vectors; HT29 Cells; HeLa Cells; Humans; Immunoglobulin G; metabolism; Immunoglobulin Heavy Chains; biosynthesis; genetics; Immunoglobulin M; metabolism; Immunoglobulin Variable Region; biosynthesis; genetics; Immunoglobulins; metabolism; RNA, Messenger; biosynthesis; genetics; Recombinant Proteins; genetics; Transfection
- From: Chinese Journal of Oncology 2006;28(2):88-91
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo determine the expression of immunoglobulins in HT-29 cells (an established colon cancer cell line, and explore their effect on the biological activities of the cancer cells.)
METHODSThe transcripts of variable regions of immunoglobulin heavy chains in HT-29 cells were detected by RT-PCR. Antisense CDR3 (specific to HT-29)-pIRES 1 neo vector was constructed, then transfected into HT-29 cells by electroporation. Programmed cell death and growth inhibition of HT-29 cells were detected by FCM and MTT, respectively.
RESULTSThe transcripts of Ig heavy chain (V(H) CDR3 region) were expressed in HT-29 cells. Moreover, they showed a monoclonal characteristic after being sequenced. After transfection of the antisense vector of CDR3 (specific to HT-29)-pIRES 1 neo, expression level of Ig in HT-29 cells was significantly decreased, and growth inhibition (P < 0.05) and apoptosis (P < 0.01) were induced.
CONCLUSIONThese results suggest that tumor derived Ig could promote the survival and growth of tumor cells.
