Effects of c-jun on hCG-induced testosterone secretion of rat Leydig cells in vitro.
- Author:
Shuanghu YUAN
1
;
Sifan XU
;
Xinhua YANG
;
Xiuqing LIU
;
Junfang HAO
;
Ming QIAN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cells, Cultured; Chorionic Gonadotropin; pharmacology; Dose-Response Relationship, Drug; Leydig Cells; secretion; Male; Oligonucleotides, Antisense; pharmacology; Proto-Oncogene Proteins c-jun; physiology; Rats; Rats, Sprague-Dawley; Testosterone; secretion
- From: National Journal of Andrology 2004;10(5):345-350
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of c-jun on hCG-induced testosterone secretion in isolated rat Leydig cells by antisense oligodeoxynucleotides(ASODNs).
METHODSc-jun ASODNs were used to antagonise the effects of c-jun, hCG was used to induce the testosterone secretion of LC cultured in vitro and testosterone was measured by radioimmunoassay.
RESULTSThe testosterone secretion of LC in vitro could be induced by hCG, which was a good model for the functional study of LC. c-jun ASODNs decreased the hCG-induced testosterone secretion of LC in a dose-dependent manner(P < 0.05).
CONCLUSIONIt is suggested that c-jun proto-oncogene enhances the testosterone secretion of LC.
