Separation of high-quality sperm by PureSperm centrifugation applied to intrauterine insemination cycles.
- Author:
Yan ZHANG
1
;
Yun QIAN
;
Ting FENG
;
Lingbo CAI
;
Jiayin LIU
Author Information
- Publication Type:Journal Article
- MeSH: Cell Separation; methods; Centrifugation, Density Gradient; Female; Humans; Insemination, Artificial; Male; Spermatozoa; cytology
- From: National Journal of Andrology 2004;10(5):348-350
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo separate high-quality sperm by PureSperm centrifugation applied to intrauterine insemination (IUI) cycles.
METHODSWe compared the separate results after washing the semen with one-layer and two-layer PureSperm gradient centrifugation methods and two-layer Percoll gradient centrifugation method, and used the recovered high-quality sperm for IUI.
RESULTSThe density of the sperm washed with one-layer PureSperm centrifugation method was significantly higher than that washed with two-layer Percoll and two-layer PureSperm centrifugation methods(P < 0.01), but there were no differences in all the results between the use of two-layer Percoll and two-layer PureSperm(P > 0.05). No significant differences in the motility, teratozoospermia and IUI results were found when the three methods were used for sperm preparation(P > 0.05). The percentage of morphologically normal sperm was markedly increased, and the non-sperm components such as leucocytes, epithelial cells and cellular fragments were significantly reduced after washed by the two methods.
CONCLUSIONPureSperm centrifugation is a safe, efficient and easy method for separating high-quality sperm on intrauterine insemination cycles.
