Association of single nucleotide polymorphism of vitamin D receptor gene start codon and the susceptibility to prostate cancer in the Han nationality in Hubei area.
- Author:
Yi YANG
1
;
Shaogang WANG
;
Zhangqun YE
;
Weimin YANG
Author Information
- Publication Type:Journal Article
- MeSH: Aged; Aged, 80 and over; China; ethnology; Codon, Initiator; Genetic Predisposition to Disease; Humans; Male; Middle Aged; Polymerase Chain Reaction; Polymorphism, Single Nucleotide; Prostatic Neoplasms; genetics; Receptors, Calcitriol; genetics
- From:National Journal of Andrology 2004;10(6):411-414
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate single nucleotide polymorphism of vitamin D receptor (VDR) gene start codon in the Han nationality in Hubei area and its relationship to the susceptibility to prostate cancer (PCa), and to study the possible mechanism for PCa.
METHODSThe VDR genotypes were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in 80 patients with PCa and 96 normal male controls from the Han nationality in Hubei area, using endonuclease Fok I. Direct sequencing was done in part of the PCR products.
RESULTSThe frequency distribution of Fok I alleles in this cohort all followed the Hardy-Weinberg equilibrium. The distribution of genotypes and alleles had no significant difference between PCa patients and normal male controls( P > 0.05).
CONCLUSIONThe results indicated no significant relationship between Fok I polymorphism of VDR gene start codon and PCa in the Han nationality in Hubei area.
