Effect of LHRH crasis protein as vaccine on the reproductive function of male rats.
- Author:
Ruifen CAI
1
;
Qinqin PAN
;
Yong PAN
;
Yue JIA
;
Dingzhi MA
;
Xinghai WANG
;
Yugui CUI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antibodies; blood; Gonadotropin-Releasing Hormone; immunology; Male; Rats; Rats, Sprague-Dawley; Recombinant Fusion Proteins; immunology; Sperm Count; Testosterone; blood; Thioredoxins; immunology; Vaccines, Synthetic; immunology
- From:National Journal of Andrology 2004;10(6):426-428
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of Trx-LHRH, a new GnRH crasis protein, on antibody production and male reproductive function.
METHODSTrx-LHRH produced in vitro with a new crasis gene which crasised Trx gene and GnRH gene together, was used as vaccine, and hydroalaminum base as adjuvant, in adult SD rats. After 5 weeks of the first treatment, the same dosage was used again to enhance the effect of vaccine. Antibody level was measured by ELISA, and androgen level by RIA.
RESULTSTrx-LHRH induced successfully the polycolonal antibody at the level of 1 :1 280 approximately 2 560 after 4 weeks of the first treatment, and 1 : 2 000 after 6 weeks of the enhanced treatment. Testosterone level was reduced significantly (P < 0.01) by ELISA, but there was reasonable variation among individuals. Sperm count was also reduced by Trx-LHRH treatment.
CONCLUSIONTrx-LHRH can be used as effective vaccine to induce antibody production, and at the same time, restrain the function of hypothatamas-pituitary-testis axis in vivo.
