Comparison of sperm motion parameters in pre-freeze and post-thaw semen samples using computer-assisted sperm analysis.
- Author:
Meiyi LIU
1
;
Shunmei DENG
;
Chunjie MA
;
Aiping CHEN
;
Yanjia JIANG
;
Renqian WEN
;
Qiling WANG
;
Lixin TANG
;
Jianchu HUANG
;
Xiaotao YAO
Author Information
- Publication Type:Journal Article
- MeSH: Cryopreservation; Diagnosis, Computer-Assisted; Freezing; Humans; Male; Sperm Motility
- From:National Journal of Andrology 2004;10(6):431-433
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe sperm motion parameters in pre-freeze and post-thaw semen samples using computer-assisted sperm analysis (CASA) system.
METHODSSemen analyses of 238 samples before freezing and after thawing were separately performed by Hamilton-Thorne Sperm Analyzer.
RESULTSSperm motility in post-thaw samples was significantly decreased. There was significant correlation and difference between pre-freeze and post-thaw samples in sperm motion parameters, including average path velocity (VAP), straight line velocity (VSL), curvilinear velocity (VCL), amplitude of lateral head displacement (ALH), straightness (STR) and linearity (LIN), except heat cross frequency (BCF). The percentage of sperm movement velocity parameters (VAP, VSL and VCL) and moving pattern parameters (ALH) significantly decreased, while that of LIN and STR significantly increased in post-thaw samples.
CONCLUSIONCASA system is of clinically applied value and is a useful tool for evaluating sperm motion parameters in pre-freeze and post-thaw semen samples.
