OBJECTIVETo compare semen analysis system of computer-aided sperm analysis (CASA) dyeing with DNA fluorescence (simplified as fluorescence CASA) with two other routine semen analyzing methods.

METHODSTwenty-two male infertile patients were selected at random as research subjects, and semen analysis was performed with fluorescence CASA, grey CASA, and microscopic analysis.

RESULTSFluorescence CASA could distinguish sperm from non-sperm granules, as well as living from dead sperm. Compared with the sperm density detected by fluorescence CASA, the disparities of sperm densities detected by microscopic analysis and grey CASA were (9.23 +/- 8.01) x 10(6)/ml and (10.27 +/- 6.22) x 10(6)/ml, and the disparity rates of sperm density were 49.06% +/- 49.87% and 43.39% +/- 25.56% respectively.

CONCLUSIONFluorescence CASA was more conformable to the requirements of latest WHO laboratory manual for the examination of human semen, and worth recommending for the clinical analysis of semen features, especially sperm density.