Protective effect of puerarin on vascular endothelial cell apoptosis induced by chemical hypoxia in vitro.
- Author:
Rui-li SHI
1
;
Jian-jun ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Aorta, Thoracic; cytology; Apoptosis; Caspase 3; Caspases; metabolism; Cattle; Cell Hypoxia; drug effects; Cells, Cultured; DNA Damage; drug effects; Endothelium, Vascular; cytology; drug effects; Isoflavones; pharmacology; Protective Agents; pharmacology
- From: Acta Pharmaceutica Sinica 2003;38(2):103-107
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo study the effect of puerarin on vascular endothelial cells apoptosis induced by chemical hypoxia-ischemia in vitro.
METHODSThe chemical hypoxia-ischemia model was performed by treating cultured bovine aortic endothelial cells (BAECs) with NaCN in glucose-free medium. Cell viability was determined by trypan blue staining. Cell apoptosis was defined by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL), flow cytometry and Hoechst 33342 staining. The expression of Caspase-3 in endothelial cells was detected by immunocytochemical method.
RESULTSChemical hypoxia-ischemia was shown to initiate bovine aortic endothelial cell apoptosis. Puerarin (0.5-3 mmol.L-1) was found to inhibit endothelial cell apoptosis effectively, and reduce the expression of Caspase-3 significantly.
CONCLUSIONPuerarin can protect apoptotic endothelial cells induced by chemical hypoxia-ischemia markedly and the effect was performed partly by decreasing Caspase-3 expression.
