Clonal expansion T cells identified in acute monoblastic leukemia by CDR3 size analysis of TCR V beta repertoire using RT-PCR and genescan.
- Author:
Yangqiu LI
1
;
Lijian YANG
;
Shaohua CHEN
;
Rongfu LI
;
Yuping ZHANG
;
Yuhong LU
;
Gengxin LUO
Author Information
- Publication Type:Journal Article
- MeSH: Complementarity Determining Regions; genetics; Genes, T-Cell Receptor beta; Humans; Leukemia, Monocytic, Acute; immunology; Reverse Transcriptase Polymerase Chain Reaction; T-Lymphocytes; immunology
- From: Chinese Medical Journal 2002;115(1):69-71
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo investigate the distribution and clonality of T cell receptor (TCR) V beta repertoire in patients with acute monoblastic leukemia (AML-M5).
METHODSExpression of the TCR V beta repertoire was analyzed using reverse transcription-polymerase chain reaction (RT-PCR), which amplified the complementarity determining region 3 of 24 TCR V beta genes in peripheral blood from 9 cases with acute myclogenous leukemia subtype 5 or acute monoblastic leukemia (AML-M5). PCR products were further studied by genescan analysis to identify T cell clonality.
RESULTSExpression of 1-10 V beta subfamilies was found in samples from 9 patients. Genescan analysis showed that some V beta subfamily products from 8 of 9 cases contained an oligoclonal peak. Oligoclonal T cells of the V beta 2 subfamily could be found in 6 patients with AML-M5.
CONCLUSIONST cell clonality expansion was found in AML-M5 cases and were tendentious in the V beta 2 subfamily, suggesting a the specific immune response for leukemia cell (M5) associated antigen and may display antileukemia activity.
