Establishment of multidrug-resistance cell line C(6)/adr and reversal of drug-resistance.
- Author:
Fusheng LIU
1
;
Chongcheng WANG
;
Qinglin ZHANG
;
Junhua LI
;
Yazhuo ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: ATP-Binding Cassette, Sub-Family B, Member 1; genetics; immunology; Animals; Antibiotics, Antineoplastic; pharmacology; Antibodies, Monoclonal; pharmacology; Cell Survival; genetics; Dose-Response Relationship, Drug; Doxorubicin; pharmacology; Drug Resistance, Multiple; genetics; Drug Resistance, Neoplasm; genetics; Erythromycin; pharmacology; Gene Expression Regulation, Neoplastic; Humans; Tumor Cells, Cultured; drug effects; Verapamil; pharmacology
- From: Chinese Medical Journal 2002;115(2):238-241
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo investigate the mechanism of multidrug resistance (MDR) in a human glioma cell and methods for overcoming multi-drug resistance.
METHODSMDR cell line C(6)/adr was established. The expression of the mdr-1 gene and its P-glycoprotein (P-gp) in the C(6)/adr cell line was observed by RT-PCR and flow cytometry. The reversal of MDR by verapamil, erythromycin, dihydropyridine, P-gp monoclonal antibody and Salvia miltiorrhiza (SM) was studied by microtiter tetrazolium (MTT) assay or by high performance liquid chromatographic assay.
RESULTSThe mdr-1 gene of the C(6)/adr cell line was positive, over-expressing P-gp. The drug-resistance of the C(6)/adr cell lines could be partly reversed by 2 - 6 microg/ml of verapamil, 50 - 100 microg/ml of erythromycin, or 5 microg/ml of dihydropyridine. As concentration increased, they had a better effect. Among these drugs, 100 microg/ml of erythromycin had the best result of reversal. Dihydropyridine 1 microg/ml, P-gp monoclonal antibody and SM had no effect.
CONCLUSIONThe mdr-1 gene and its expression might be associated with the MDR of glioma cells. Verapamil, erythromycin and dihydropyridine could reverse the MDR of glioma cells.
