Expression and effect of basic fibroblast growth factor on human cataract lens epithelial cells.
- Author:
Xingchao SHENTU
1
;
Ke YAO
;
Chaohui SUN
;
Wen XU
;
Renyi WU
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Age Factors; Cataract; metabolism; pathology; Child; Child, Preschool; Epithelial Cells; chemistry; pathology; Fibroblast Growth Factor 2; biosynthesis; Humans; Immunohistochemistry; Infant; Infant, Newborn; Lens, Crystalline; chemistry; pathology; Middle Aged; Proliferating Cell Nuclear Antigen; analysis
- From: Chinese Medical Journal 2002;115(2):268-271
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo detect the expression of basic fibroblast growth factor (bFGF) in human ocular tissues, and to assess the effect of bFGF on the proliferation of human cataract lens epithelial cells (LECs) and its correlation with age.
METHODSEnucleated eyes were subjected to immunostaining for bFGF protein. Human cataract LECs were cultured in vitro, and treated with bFGF for 48 hr. Proliferation was estimated by the positive area ratio of proliferating cell nuclear antigen (PCNA) in immunohistochemistry.
RESULTSbFGF protein was found in various human ocular tissues. bFGF stimulated human cataract LEC proliferation, and there was an age-related decrease in responsiveness of human cataract LECs to bFGF (P < 0.05).
CONCLUSIONbFGF might play an important role in the proliferation of residual human cataract LECs after cataract surgery.
