Effects of shenmai injection on expression of TNF-alpha mRNA in peritoneal macrophages of scald mice.
- Author:
Runtian WANG
1
;
Chao GAO
;
Dianwu LIU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Burns; genetics; mortality; Drugs, Chinese Herbal; pharmacology; Gene Expression Regulation; drug effects; Macrophages, Peritoneal; cytology; drug effects; metabolism; Mice; Mice, Inbred BALB C; RNA, Messenger; drug effects; genetics; metabolism; Survival Rate; Time Factors; Tumor Necrosis Factor-alpha; genetics
- From: Chinese Medical Journal 2002;115(2):293-295
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo explore the effect of shenmai injection (SI) on expression of TNF-alpha mRNA in peritoneal macrophages (pMPhis) of scald mice.
METHODSBALB/c mice were inflicted with 11% of body surface area III degree scald and injected intraperitoneally (ip) with SI daily for 5 days, and expression of TNF-alpha mRNA in pMPhis was determined by semi-quantitative RT-PCR.
RESULTSIn scald mice, the expression of TNF-alpha mRNA in pMPhis increased significantly, but it was reduced obviously (P < 0.01) after SI administration, while the livability was increased markedly (P < 0.05).
CONCLUSIONSFor scald mice, the cause of death at early stage might be related to the high expression of TNF-alpha mRNA in pMPhis and the use of SI can decrease the death rate.
