Association of the phosphatidylinositol signal pathway with prolonged myocardial ischemia.
- Author:
Xiuyun DING
1
;
Jiachang YUE
;
Shiwen WANG
;
Xue GAO
;
Xianfeng LI
Author Information
- Publication Type:Journal Article
- MeSH: 1-Phosphatidylinositol 4-Kinase; metabolism; Animals; Male; Myocardial Ischemia; enzymology; Phosphotransferases (Alcohol Group Acceptor); metabolism; Protein Kinase C; metabolism; Random Allocation; Rats; Rats, Wistar; Signal Transduction
- From: Chinese Medical Journal 2002;115(3):367-370
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo study the changes in activity of phosphatidylinositol 4 kinase (PI 4 kinase), phosphatidylinositol 4 phosphate 5 kinase (PIP 5 kinase) and protein kinase C (PKC) during myocardial ischemia and elucidate the relationship between phosphatidylinositol signal pathways and prolonged myocardial ischemia.
METHODSIn vivo an ischemic rat model was used. Activity of PI 4 kinase, PIP 5 kinase and PKC were measured at different times in postischemic heart cells using isotope analysis.
RESULTSThe activity of PI kinase, PIP kinase and PKC in the myocardium increased to peak at 1 hour postischemia, with activities 6.1, 3.0 and 4.0 fold over control levels, respectively. Their activities declined to normal levels with time.
CONCLUSIONThe phosphatidylinositol signal pathway is involved in prolonged myocardial ischemia, but its mechanism needs further study.
