Helicobacter pylori antigen and its IgG, IgA -type specific immunocomplexes in sera from patients with Helicobacter pylori infection.
- Author:
Yongliang ZHU
1
;
Jie LIN
;
Da LI
;
Qin DU
;
Keda QIAN
;
Qindong WU
;
Shu ZHENG
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Antibody Specificity; Antigen-Antibody Complex; blood; Antigens, Bacterial; blood; Female; Helicobacter Infections; immunology; Helicobacter pylori; immunology; Humans; Immunoglobulin A; blood; Immunoglobulin G; blood; Male
- From: Chinese Medical Journal 2002;115(3):381-383
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo explore the characteristics of Helicobacter pylori (H. pylori) antigen in serum and to evaluate its clinical diagnostic value.
METHODSEnzyme-linked immunosorbant assay (ELISA) was developed to detect the soluble H. pylori antigen (S-Hp) and circulatory specific H. pylori antigen immunocomplexes (Hp-IC) in serum.
RESULTSThe positive rate of S-Hp was 90.91% from 66 patients with H. pylori infection, which was much greater than 0% found in 28 controls (P < 0.001). Moreover, its concentration closely reflected the number of H. pylori in the gastric mucosa layer. We also found that Hp-IC existed bound with IgG and/or IgA in patients with positive S-Hp. However, there is no evidence to show the concentration of S-Hp reduced significantly in followed-up subjects after effective therapy.
CONCLUSIONSThese methods as newly and noninvasive complementary tools can be used for clinical diagnosis of H. pylori infection. In addition, S-Hp and Hp-IC may be of importance in H. pylori pathogenesis.
