Mutations in exon 19 of EGFR gene in non-small cell lung cancer from Chinese patients.
- Author:
He-yong WANG
1
;
Hen LUO
;
Yin-min ZHAO
;
Liang TANG
;
Cai-cun ZHOU
Author Information
- Publication Type:Journal Article
- MeSH: Adenocarcinoma; genetics; Adult; Aged; Aged, 80 and over; Carcinoma, Non-Small-Cell Lung; genetics; DNA Mutational Analysis; Exons; genetics; Female; Genes, erbB-1; genetics; Humans; Male; Middle Aged; Mutation; Polymerase Chain Reaction; Sex Factors; Smoking
- From: Chinese Journal of Medical Genetics 2008;25(3):315-318
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the mutations in exon 19 of epidermal growth factor receptor (EGFR) gene in non-small cell lung cancer from Chinese patients.
METHODSGenomic DNA was extracted from 72 lung cancer tissues. Then the exon 19 of EGFR gene was amplified by nested PCR and sequenced.
RESULTSIn 13 tumor tissues, multi-nucleotide in-frame deletion mutations at the exon 19 of EGFR gene, had been detected. There were 4 mutation types. The mutation rate was 18.1%. The mutations were all heterozygous. There was association of the exon 19 mutation of EGFR gene with adenocarcinoma, female patients and non-smokers.
CONCLUSIONThere were multi-nucleotide in-frame deletion mutations in exon 19 of EGFR gene. Mutations of the exon 19 of EGFR gene were higher in female, non-smoking and adenocarcinoma patients.
