Assay of the expression and self-activition function of full length and different fragments of mayven gene in yeast two-hybrid system.
- Author:
Fang LIU
1
;
Zhiyin DU
;
Jun-lin HE
;
Ying-xiong WANG
Author Information
- Publication Type:Journal Article
- MeSH: Microfilament Proteins; genetics; Models, Genetic; Multiple Sclerosis; genetics; Nerve Tissue Proteins; genetics; Polymerase Chain Reaction; Two-Hybrid System Techniques
- From: Chinese Journal of Medical Genetics 2008;25(3):338-342
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo identify the mayven-interacting proteins and study the effect of mayven on multiple sclerosis pathogenesis.
METHODSThe full length and four different fragments of mayven gene were amplified by polymerase chain reaction (PCR) with specific primers and were cloned into the yeast expression vector pDBLeu. Then, the recombinant plasmids were transformed into MAV203 yeast strain. The autonomous activation of their expression products was detected.
RESULTSThe yeast expression vectors of mayven, which include a full-length and four different fragments, were constructed successfully. Full length P1, fragments P3 and P4 have no effect on the expression of HIS3 and LacZ gene, but fragments P7 and P8 do. The C-terminal of Mayven gene may contain a transcription activation domain.
CONCLUSIONFull length P1, fragments P3 and P4 of the mayven gene can be used to screen the mayven-interacting proteins, but whether Mayven has transcriptional activation activity need to be studied.
