Association of genetic polymorphisms and haplotypes in hMLH1 and hMSH3 gene with the risk of papillary thyroid carcinoma.
- Author:
Wen-ping SHI
1
;
Jian-chao BIAN
;
Feng JIANG
;
Hong-xia NI
;
Qian-xi ZHU
;
Hong-wei TANG
;
Qiang SHEN
;
Yi WU
Author Information
- Publication Type:Journal Article
- MeSH: Adenocarcinoma, Papillary; genetics; Adult; Female; Genetic Predisposition to Disease; Haplotypes; genetics; Humans; Male; Middle Aged; Polymerase Chain Reaction; Polymorphism, Genetic; Polymorphism, Single Nucleotide; Risk; Thyroid Neoplasms; genetics
- From: Chinese Journal of Medical Genetics 2008;25(4):390-395
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the relationship of the genetic polymorphisms and the haplotypes in hMLH1 and hMSH3 gene with the risk of papillary thyroid carcinoma (PTC) in Chinese Hans.
METHODSA hospital based 1:1 matched case-control study was carried out. The polymorphisms for 204 pairs of PTC cases and healthy controls were identified by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and allele specific oligonucleotide (PCR-ASO) assays.
RESULTS(1) The PTC risk was marginally increased in the hMLH1 1151TA genotype, with odds ratio (OR) of 2.15 (95%CI: 0.99-4.85); the PTC risk was significantly increased in the mutant genotype 1151TA+AA, with OR of 2.15 (95%CI: 1.02-4.69); (2) The haplotypes of -93G, 1151A, 655A in the hMLH1 gene could increase the PTC risk, with OR of 2.67 (95%CI: 1.16-6.53, P=0.011), compared with the haplotype of -93G, 1151T, 655A; (3) Compared to 3124A, 2835G haplotype in hMSH3 gene, the 3124G, 2835A haplotype could increase the PTC risk marginally, with OR of 3.08 (95%CI: 0.92-13.25).
CONCLUSIONThe 1151T/A polymorphism in hMLH1 was associated with PTC; both the haplotype of -93G, 1151A, 655A in hMLH1 and the 3124G, 2835A haplotype in hMSH3 were associated with PTC.
