Sequence analysis of a novel human leukocyte antigen allele HLA-A*9206.
- Author:
Jun-jun HE
1
;
Wei ZHANG
;
Wei WANG
;
Zhe-dong HAN
;
Nan-ying CHEN
;
Fa-ming ZHU
;
Li-xing YAN
Author Information
- Publication Type:Journal Article
- MeSH: Alleles; Asian Continental Ancestry Group; Base Sequence; HLA-A Antigens; genetics; Humans; Molecular Sequence Data; Polymerase Chain Reaction; Sequence Alignment; Sequence Analysis; Sequence Analysis, DNA; Sequence Homology, Nucleic Acid
- From: Chinese Journal of Medical Genetics 2008;25(4):396-399
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the molecular genetic basis for a human leukocyte antigen (HLA) novel allele HLA-A*9206 in the Chinese population.
METHODSDNA was extracted from whole blood by PEL-FREEZ DNA extraction kit. The amplification of HLA-A exons 1-8 of the proband was preformed and the PCR products were sequenced using ABI sequencing kit. Both strands of exons 2, 3 and 4 of the amplified product were sequenced. The polymerase chain reaction-sequence specific primer (PCR-SSP) was performed to split the two alleles apart and confirm the mutations detected by sequencing.
RESULTSThe sequencing results showed that the HLA-A alleles of the proband were A*1101 and a novel allele. The sequence of the novel allele has been submitted to GenBank (EF062306). After Blast analysis, the novel allele shows one nucleotide different from the HLA-A*0206 in exon 3 at nucleotide position 530 (C to T). This results in an amino acid change from Ala to Val at codon 153.
CONCLUSIONThis allele is a novel allele and has been officially named A*9206 by the WHO Nomenclature Committee.
