Study of the HLA-A, B, DRB1 allele and haplotype frequencies of 4194 cord blood samples in Guangzhou.
- Author:
Wen-qiong SHA
1
;
Can LIAO
;
Yan LI
;
Xin YANG
;
Xue-wei TANG
;
Yi-ning HUANG
;
Xing-mei XIE
Author Information
- Publication Type:Journal Article
- MeSH: Alleles; Asian Continental Ancestry Group; genetics; China; Female; Gene Frequency; Genetics, Population; HLA-A Antigens; genetics; HLA-B Antigens; genetics; HLA-DR Antigens; genetics; Haplotypes; Humans
- From: Chinese Journal of Medical Genetics 2008;25(4):473-476
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo analyze the distribution of the human leukocyte antigen (HLA)-A, B and DRB1 allele and haplotype in cord blood samples preserved in Guangzhou Cord Blood Bank collected in the last 10 years.
METHODSThe HLA-A, B and DRB1 genotyping of 4194 cord blood samples were detected by Special Monoclonal Tray, PCR-sequence specific promer (PCR-SSP), PCR-sequence specific oligonucleotide probe (PCR-SSO) and sequence based typing (SBT). Frequencies of HLA-A, B and DRB1 allele and haplotype were calculated by Arlequin software.
RESULTSThe total numbers of HLA-A, B and DRB1 alleles are 18, 43, 13 respectively. The obviously high frequency alleles are A*11, A*02, A*24, A*33, B*40, B*15, B*46, B*13, DRB1*12, DRB1*15, DRB1*09 and DRB1*04, with accumulative frequency of each locus being more than 50%. The most common haplotypes are A2-B46, B46-DR9, A11-DR12 and A2-B46-DR9.
CONCLUSIONThe distribution of HLA-A, B and DRB1 allele and haplotype of cord blood in Guangzhou Cord Blood Bank has typical characteristics of southern Chinese Han population. Authors' data may help in searching for appropriate donors.
