The mechanism of apoptosis in human U87 glioma cells induced by miR-21 antisense oligonucleotide.
- Author:
Lei SHI
1
;
Zhihao CHENG
;
Junxia ZHANG
;
Rui LI
;
Yongping YOU
;
Zhen FU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Apoptosis; drug effects; genetics; Caspases; metabolism; Cell Line, Tumor; Cell Proliferation; Enzyme Activation; genetics; Gene Expression Regulation, Neoplastic; Glioma; genetics; pathology; Humans; MicroRNAs; antagonists & inhibitors; genetics; Oligoribonucleotides, Antisense; genetics; pharmacology; PTEN Phosphohydrolase; metabolism; Transfection
- From: Chinese Journal of Medical Genetics 2008;25(5):497-501
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the mechanism of U87 cell apoptosis induced by inhibiting miR-21 expression.
METHODSAntisense oligonucleotides of miR-21 were chemically synthesized and transfected into U87 cells. The apoptosis, proliferation, and invasion of the cells were evaluated. The relationship between miR-21 and PTEN or caspase was identified by bioinformatics and Western blot.
RESULTSInhibiting miR-21 expression led to U87 cell growth suppression, apoptosis induction, invasion reduction, caspase-3 activity elevation and caspase-9 activation, but did not affect PTEN and caspase-8 expression.
CONCLUSIONmiR-21 may function as an antiapoptotic miRNA in U87 cells. Inhibiting miR-21 expression could induce U87 cell apoptosis via caspase-9 and 3 activation, but not PTEN activation.
