Association of matrix metalloproteinase-3 serum level and the promoter 5A/6A polymorphism of the MMP-3 gene with atherosclerotic cerebral infarction.
- Author:
Xiaoya HUANG
1
;
Min ZHU
;
Xiaoping JIN
;
Danhong ZHANG
;
Linzhi WANG
;
Zusen YE
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; Aged, 80 and over; Asian Continental Ancestry Group; genetics; Case-Control Studies; Cerebral Infarction; blood; complications; genetics; Ethnic Groups; genetics; Female; Gene Frequency; Genotype; Humans; Intracranial Arteriosclerosis; blood; complications; genetics; Male; Matrix Metalloproteinase 3; blood; genetics; Middle Aged; Polymorphism, Genetic; Promoter Regions, Genetic; genetics
- From: Chinese Journal of Medical Genetics 2008;25(6):653-656
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the association of matrix metalloproteinase-3 (MMP-3) serum level and the promoter 5A/6A polymorphism of the MMP-3 gene with atherosclerotic cerebral infarction (ACI) in a Chinese Han population.
METHODSTwo hundred and fifteen patients with acute ACI from the Department of Neurology of Taizhou Hospital and 226 healthy controls were included in the study. Serum MMP-3 level was measured by enzyme-linked immunosorbent assay (ELISA). Genotype was determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) for the common 5A/6A functional promoter polymorphism of the MMP-3 gene.
RESULTSThe genotype distribution of the MMP-3 promoter 5A/6A polymorphism between the ACI patients group and the control group was significantly different (chi (2)= 9.389, P= 0.002). The frequencies of the 5A allele were 14.2% and 7.7% in the ACI patients group and the control group respectively (chi (2)= 9.430, P= 0.002). Serum level of MMP-3 in the ACI patients group was significantly higher than that in the control group (t= 24.867, P= 0.000). Among the ACI patients group, serum MMP-3 levels also had significant difference between the 5A/6A+ 5A/5A and the 6A/6A genotype (t= 2.057, P= 0.041).
CONCLUSIONThe present findings suggest that serum level of MMP-3 obviously increased within 48 hours of ischemic stroke and the genetic polymorphism of 5A/6A in the MMP-3 promoter is associated with ACI and MMP-3 expression in the Chinese Han population.
