UbcH10 expression in hepatocellular carcinoma and its clinicopathological significance.
- Author:
Shao-shan HAN
1
;
Qing-guang LIU
;
Ying-min YAO
;
Hao SUN
;
Xian-feng ZAN
;
Tao SONG
;
Xue YANG
;
Xin ZHENG
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; Biomarkers, Tumor; Carcinoma, Hepatocellular; metabolism; pathology; Female; Hep G2 Cells; Humans; Liver Neoplasms; metabolism; pathology; Male; Middle Aged; RNA, Messenger; genetics; metabolism; Ubiquitin-Conjugating Enzymes; genetics; metabolism
- From: Journal of Southern Medical University 2011;31(2):280-284
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate UbcH10 expression in hepatocellular carcinoma and explore its clinicopathological implications.
METHODSWe detected UbcH10 mRNA expression using RT-PCR in normal liver cell line, cancer cell lines, surgically removed hepatocellular carcinoma tissue and corresponding adjacent non-tumor tissue and evaluated the clinicopathological significance of UbcH10. Immunohistochemistry was performed to investigate UbcH10 protein expression in hepatocellular carcinoma tissue, the adjacent tissue, and normal liver tissue specimens.
RESULTSNormal liver cell line L02 showed significantly lower UbcH10 mRNA expression levels than the cancer cell lines BEL-7402, Hep3B, HepG2 and SMMC-7721 (P<0.05). UbcH10 mRNA expression was also was significantly higher in hepatocellular carcinoma tissues than in the corresponding non-tumor tissues (P<0.05). Clinicopathological evaluation suggested that UbcH10 expression was associated with tumor invasion of the portal vein, tumor size, TNM staging, and tumor differentiation (P<0.05). Immunohistochemistry identified stronger UbcH10 expression in hepatocellular carcinoma tissues than in the adjacent tissues and normal liver tissues (68.6%, 28.6%, and 26.7%, respectively).
CONCLUSIONUbcH10 is over-expressed in hepatocellular carcinoma and may serve as a novel biomarker as well as a therapeutic target of hepatocellular carcinoma.
