Isolation of a wild-type virulent phage of Helicobacter pylori and its simulated treatments of gastrointestinal Hp in vitro.
- Author:
Xue-qin WAN
1
;
Dong-sheng TANG
;
Ai-ping LIU
;
Shu-yi TAN
;
Wan-kelan LI
;
Jia KUANG
;
Hong-ming LI
Author Information
- Publication Type:Journal Article
- MeSH: Bacteriophages; isolation & purification; physiology; Gastrointestinal Tract; microbiology; Helicobacter Infections; therapy; Helicobacter pylori; virology; Humans; Virulence
- From: Journal of Southern Medical University 2011;31(2):304-307
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo isolate the wild-type virulent phage of Helicobacter pylori (Hp) and simulate the treatments in vitro to investigate the methods for oral Hp-assisted penetration of the phage through the gastric barrier and offspring phage release for infection and treatment of gastrointestinal Hp.
METHODSThe Hp strain was cultured with the candle cylinder method and the virulent phage was isolated by single plate or double plate experiment. A simulated gastric juice was applied and the bactericidal effect of the phage was tested with double flats experiment.
RESULTSAfter a 1.5-h treatment in simulated gastric juice, the orally derived Hp-borne phage was still capable of forming plaques while the control phage was not.
CONCLUSIONThe oral Hp can help the phage resist the gastric juice and then infect the gastrointestinal Hp.
