Expression of miR-146a and miR-16 in peripheral blood mononuclear cells of patients with rheumatoid arthritis and their correlation to the disease activity.
- Author:
Zhi-tao FENG
1
;
Juan LI
;
Jie REN
;
Zhuo LV
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Arthritis, Rheumatoid; blood; Biomarkers; Female; Humans; Leukocytes, Mononuclear; metabolism; Male; MicroRNAs; genetics; metabolism; Middle Aged; Young Adult
- From: Journal of Southern Medical University 2011;31(2):320-323
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the expression levels of miR-146a and miR -16 in the peripheral blood mononuclear cells (PBMCs) of patients with rheumatoid arthritis (RA) and their correlation to the disease activity.
METHODSThe expression levels of miR-146a and miR-16 in the in PBMCs were measured using real-time RT-PCR in 40 RA patients and 16 healthy individuals. The correlations of miR-146a and miR-16 expressions to the disease activity of RA were analyzed.
RESULTSThe expression levels of miR-146a and miR-16 in the PBMCs of RA patients increased significantly compared to those in the healthy individuals (P<0.05). Their expression levels was significantly higher in active RA patients than in patients with disease remission (P<0.05) and healthy individuals (P<0.01). The expression levels of miR-146a and miR-16 were positively correlated to ESR, CRP and the disease activity scores in 28 joints (DAS28) (P<0.01), but not to RF (P>0.05).
CONCLUSIONThe elevated expression levels of miR-146a and miR-16 are correlated to RA disease activity, suggesting their value in assessment of the clinical disease activity of RA.
