Association between cell apoptosis and the quality of early mouse embryos.
- Author:
Min YU
1
;
Zhuo-lin QIU
;
Hong LI
;
Wei-sen ZENG
;
Lei-Ning CHEN
;
Qiu-hua LI
;
Song QUAN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Apoptosis; Caspases; metabolism; Embryo, Mammalian; cytology; Female; Mice; Mice, Inbred Strains; Pregnancy; Proliferating Cell Nuclear Antigen; metabolism; Proto-Oncogene Proteins; metabolism; Proto-Oncogene Proteins c-bcl-2
- From: Journal of Southern Medical University 2011;31(3):409-413
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the relationship between cell apoptosis and the quality of early mouse embryos, understand the significance of apoptosis-regulatory genes in early embryonic development, and explore a new approach to improving the embryo quality.
METHODSThe levels of cell apoptosis and proliferation in early mouse embryos in different developmental status (morphologically normal embryos, arrested embryos and fragmented embryos) were analyzed with terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL), caspase in situ fluorescence and Bcl-2 immunofluorescence, and immunofluorescent detection of proliferating cell nuclear antigen (PCNA).
RESULTSThe cells in arrested embryos and embryonic fragments showed positive results in TUNEL assay with enhanced caspase activity and lowered expressions of Bcl-2 and PCNA.
CONCLUSIONCell apoptosis in early mouse embryos may be closely related to embryonic arrest and fragmentation.
