Establishment of a nasopharyngeal carcinoma cell line CNE1 stably overexpressing ebv-miR-BART7 mediated by lentivirus.
- Author:
Cun-cun YUAN
1
;
Hong-bing CAI
;
Yu-qi HUANG
;
Yan-fen YE
;
Man-li ZHAO
;
Xiao-ming LÜ
;
Xin LI
Author Information
- Publication Type:Journal Article
- MeSH: Carcinoma; Cell Line, Tumor; Genetic Vectors; Humans; Lentivirus; genetics; MicroRNAs; Nasopharyngeal Neoplasms; genetics; Plasmids
- From: Journal of Southern Medical University 2011;31(3):419-422
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a nasopharyngeal carcinoma (NPC) cell line CNE1-pLVTHM/BART7 with stable ebv-miR-BART7 overexpression.
METHODSThe recombinant lentivirus pLVTHM/BART7 expression plasmid was packaged into mature lentivirus by 293FT cells and used to infect CNE1 cells. Flow cytometry was employed for sorting the GFP(+) cells. The efficiency of ebv-miR-BART7 overexpression was determined using qRT-PCR.
RESULTSThe recombinant lentivirus plasmid pLVTHM/BART7 was successfully constructed and verified by PCR and sequencing. The expression of ebv-miR-BART7 in CNE1 cells infected with the lentivirus pLVTHM/BART7 was significantly increased as compared with the negative control and the blank control cells.
CONCLUSIONThe recombinant lentivirus vector pLVTHM/BART7 results in high and stable expression of ebv-miR-BART7 in infected CNE1 cells, which provides a useful cell model for further studies of the role of ebv-miR-BART7 in nasopharyngeal carcinoma.
