Gene expression profiling of nasopharyngeal carcinoma cell line 5-8F-EGFP and the liver metastatic 5-8F-H3B-EGFP cells.
- Author:
Chun HAN
1
;
Jun-lan YANG
;
Kai-tai YAO
Author Information
- Publication Type:Journal Article
- MeSH: Carcinoma; Cell Line, Tumor; Gene Expression Profiling; Gene Expression Regulation, Neoplastic; Humans; Liver Neoplasms; genetics; secondary; Nasopharyngeal Neoplasms; genetics; pathology; Neoplasm Metastasis; Oligonucleotide Array Sequence Analysis; Transcriptome
- From: Journal of Southern Medical University 2011;31(3):473-477
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo compare the gene expression profiles of nasopharyngeal carcinoma (NPC) cell line 5-8F-EGFP and the liver metastatic 5-8F-H3B-EGFP cells.
METHODSThe fluorescence-labeled cDNA were prepared separately from the total RNA extracted from the two cell lines and hybridized with Human_U133A2.0 Genechip (Affymetrix, USA) containing approximately 18 400 known gene. The gene expression profiles were analyzed with special software and cluster analysis.
RESULTSA total of 3767 genes were identified to have significant differential expressions between these two cell lines (P<0.05), among which 281 genes showed twofold or higher differential expressions. Using MILANO software, we found 16 genes with probable close relation with liver metastasis of NPC.
CONCLUSIONThe 16 genes differentially expressed between the two cell lines can be of importance in the investigation of the molecular mechanism of NPC liver metastasis and identification of molecular markers for prognostic evaluation.
