Effect of HIV-1gp41 ectodomain on Cryptococcus neoformans-induced cytoskeletal changes in human brain microvascular endothelial cells.
- Author:
Min LONG
1
;
Hong CAO
;
Ambrose JONG
Author Information
- Publication Type:Journal Article
- MeSH: Brain; blood supply; Cells, Cultured; Cryptococcosis; pathology; Cryptococcus neoformans; pathogenicity; Cytoskeleton; drug effects; metabolism; Endothelial Cells; cytology; drug effects; microbiology; Endothelium, Vascular; cytology; drug effects; metabolism; HIV Envelope Protein gp41; pharmacology; Humans; Microcirculation
- From: Journal of Southern Medical University 2011;31(3):478-481
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of HIV-1 gp41 ectodomain (gp41-I90) on the cytoskeletal changes in human brain microvascular endothelial cells (HBMECs) induced by Cryptococcus neoformans.
METHODSHBMECs were cultured on collagen-coated chamber slide or transwell to allow the formation of cell monolayers. After pre-treatment with gp41-I90 and infection with Cryptococcus neoformans, the HBMECs were examined for the expression of actin or filamin by immunofluorescence assay. HRP permeability of the HBMECs treated with gp41-I90 was detected by ELISA. Transcytosis of Cryptococcus neoformans through the gp41-I90-treated HBMECs was detected by direct counting from a hemocytometer.
RESULTSgp41-I90 obviously enhanced the cytoskeletal changes of the HBMECs infected by Cryptococcus neoformans, causing curved and sparse filamentous arrangement of actin and filamin. gp41-I90 treatment also resulted in obviously increased HRP permeability of the cells and transcytosis of Cryptococcus neoformans.
CONCLUSIONgp41- I90 enhances Cryptococcus neoformans binding to HBMECs, which is related to its effect in enhancing Cryptococcus neoformans-induced cytoskeletal changes of the cells.
