Characterization of specific monoclonal antibodies to Aspergillus conidia by flow cytometry.
- Author:
Nan YU
1
;
Xiao-peng YUAN
;
Jing JIN
;
Wei HAO
;
Yan-fang WANG
;
Xiao-yan CHE
Author Information
- Publication Type:Journal Article
- MeSH: Antibodies, Fungal; immunology; isolation & purification; Antibodies, Monoclonal; immunology; isolation & purification; Antibody Specificity; Aspergillus; immunology; Flow Cytometry; Spores, Fungal; immunology
- From: Journal of Southern Medical University 2011;31(3):487-489
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo characterize the specific monoclonal antibodies to Aspergillus conidia.
METHODSFlow cytometry was used to examine the reactivity of the specific monoclonal antibodies to Aspergillus conidia.
RESULTSBoth the monoclonal antibodies MA3 and Con2 showed specific reactivity to Aspergillus conidia suspensions. MA3 was capable of binding to the conidia of A.fumigatus, A.flavus, A.niger and A.terreus, while Con2 was reactive only to the conidia of A.fumigatus.
CONCLUSIONTwo specific monoclonal antibodies (MA3 and Con2) to Aspergillus conidia have been obtained.
