Effect of platelet-rich plasma on the proliferation and adipogenic differentiation of human adipose-derived stem cells in vitro.
- Author:
Yun-song ZHANG
1
;
Jing-hua HE
;
Guan-ying XIAO
;
Qing-mei LI
Author Information
- Publication Type:Journal Article
- MeSH: Adipocytes; cytology; Adipose Tissue; cytology; Cell Culture Techniques; Cell Differentiation; Cell Proliferation; Cells, Cultured; Culture Media, Conditioned; Humans; Lipectomy; Platelet-Rich Plasma; Stem Cells; cytology; Tissue Engineering; methods
- From: Journal of Southern Medical University 2011;31(3):525-528
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the effect of platelet-rich plasma (PRP) on the proliferation and adipogenic differentiation of human adipose-derived stem cells in vitro.
METHODSHuman adipose-derived stem cells were obtained by enzymatic digestion and PRP was prepared by dual centrifugal method. The ADSCS were interfused with 5%, 10%, and 20% PRP in conditioned culture media, using the untreated cells as the control group. The morphology of the cells were observed and their proliferative ability was detected using XTT colorimetric assay. The adipogenic differentiation ability of the cells was evaluated using oil Red O staining.
RESULTSThe ADSCS treated with PRP showed better morphology with higher density than the control cells. XTT colorimetric assay demonstrated obviously stronger proliferative activity of PRP-treated cells than the control group (P<0.01). Interfusion with PRP caused a significant increase in adipogenic differentiation of the cells as compared to the control cells (P<0.01).
CONCLUSIONPRP treatment produces obvious effects on the proliferation and adipogenic differentiation of human adipose-derived stem cells in vitro.
