Transmission disequilibrium test for 15 short tandem repeat loci in Kashin-Beck disease and their interaction with low selenium.
- Author:
Xiao-Wei SHI
1
;
Ai-Li LV
;
Feng-Ling REN
;
Wen-Rong LI
;
Long-Li KANG
;
Xiong GUO
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Alleles; Child; Female; Gene Frequency; Genetic Predisposition to Disease; Genotype; Humans; Kashin-Beck Disease; blood; etiology; genetics; Male; Microsatellite Repeats; Middle Aged; Pedigree; Selenium; blood; Young Adult
- From: Journal of Southern Medical University 2011;31(4):567-571
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo identify the genetic susceptibility to Kashin-Beck disease (KBD) and explore the interaction between low selenium (Se) and the susceptibility gene loci in KBD.
METHODSThe DNA samples collected from 23 KBD nuclear families were analyzed using PCR and GeneScan Analysis 3.7 and Genotyper3.7 software. The haplotype relative risk (HRR) and transmission disequilibrium test (TDT) were used to test the data of the genotypes. The serum selenium (Se) concentration was measured by atomic fluorescence spectrometry, and the interaction between low Se and the susceptibility loci was calculated using a binary logistic regression.
RESULTSIn the 23 nuclear families, the alleles of D2S151 (248 bp), D2S305 (320 bp), and D11S4094 (194 bp) showed significant correlation to KBD (P<0.05). Serum Se concentrations in the studied individuals was 0.037 µg/ml. No significant statistical interaction was observed between low Se exposure and the susceptibility loci (P>0.05).
CONCLUSIONThe polymorphisms in the STR loci D2S305, D2S151, and D11S4094 or the polymorphism loci near them might been related to KBD susceptibility. Low Se exposure shows no significant interaction with the susceptibility loci.
