Effect of total flavonoids of jiawei wuzi yanzong prescription on VGCCs induced by amyloid-beta25-35 peptide in CA1 pyramidal neurons of rat hippocampal slice.
- Author:
Li LI
1
;
Haoran CAI
;
Lin LI
;
Xuemei WANG
Author Information
- Publication Type:Journal Article
- MeSH: Alzheimer Disease; drug therapy; metabolism; physiopathology; Amyloid beta-Peptides; metabolism; Animals; CA1 Region, Hippocampal; cytology; drug effects; physiology; Calcium Channels; genetics; physiology; Disease Models, Animal; Drugs, Chinese Herbal; chemistry; pharmacology; Electrophysiology; Female; Flavonoids; chemistry; pharmacology; Humans; Male; Peptide Fragments; metabolism; Prescription Drugs; chemistry; pharmacology; Pyramidal Cells; drug effects; physiology; Rats; Rats, Sprague-Dawley
- From: China Journal of Chinese Materia Medica 2009;34(15):1975-1978
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the effect of the total flavonoids of Jiawei Wuzi Yanzong prescription on the Voltage-gated calcium channel of the CA1 pyramidal cell of rat hippocampus.
METHODThe inward calcium current was recorded by the whole-cell patch clamp and the amplitude of it was thought to observe the effect of Abeta25-35 and the total flavonoids. The hippocampus of rat was separated and cut into slices. Active pyramidal cells of slices were chosen for the whole-cell patch clamp recording. After exposure to Abeta25-35, voltage steps (500 ms) were used to depolarize stepwise in a range from 50 to +50 mV (increment: 5 mV). An inward Ca2+ current which was suggested to be survey was evoked. Application of the total flavonoids was to be observed if it had effect on this voltage-depended inward current.
RESULTAbeta25-35 could enhance the calcium current to induce intracellular calcium overload. The amplitude of the control group was--(157.1 +/- 19.9) pA, but after application of Abeta25-35 the current enhanced to--(323.2 +/- 23.4) pA. When the total flavonoids at concentration of 125, 250, 500 g x L(-1) were added, the current declined to--(257.9 +/- 31.6), - (196.4 +/- 29.8) and--(169.3 +/- 34.0) pA, respectively.
CONCLUSIONAbeta-induced intracellular calcium overload may be one of neurotoxic of beta-amyloid peptide. The total flavonoids of Jiawei Wuzi Yanzong prescription can suppress inward calcium current to protect neurons.
