Affects of different bioreactors in process of Salvia miltiorrhiza adventitious root culture.
- Author:
Shuo YAN
1
;
Wenyuan GAO
;
Juan WANG
;
Tao HUANG
;
Yu CAO
Author Information
- Publication Type:Journal Article
- MeSH: Biomass; Bioreactors; Culture Media; chemistry; metabolism; Plant Extracts; chemistry; Plant Roots; chemistry; growth & development; metabolism; Salvia miltiorrhiza; chemistry; growth & development; metabolism; Tissue Culture Techniques; methods
- From: China Journal of Chinese Materia Medica 2009;34(16):2027-2030
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the affects of the different bioreactors on the Salvia miltiorrhiza adventitious root culture.
METHODAdventitious roots of S. miltiorrhiza were induced and in vitro cultured in bioreactors. The type of bioreactors was optimized and the kinetics of root growth, accumulation of the active ingredients was also investigated by HPLC.
RESULTIt showed that the 3-1 conical bubble bioreactor (CNBB) with 60 degrees taper favors achieved the highest active compounds accumulation in adventitious roots. The growth curve and secondary metabolites accumulation curve looks like "S" in CNBB bioreactor. The maximum adventitious roots biomass of 16.24 g x L(-1) (fresh weight) was obtained at 35 day. The highest content of tanshinone IIA (TA) and protocatechuic aldehyde (PA) reached 0.23 mg x g(-1) DW and 0.51 mg x g(-1) DW at 40 day, respectively.
CONCLUSIONThe 3-1 conical bubble bioreactor (CNBB) with 60 degrees taper favors was the best bioreactors for the accumulation of active compounds.
