Simultaneous determination of six flavonoids in Flos Chrysanthemi Indici by RP-HPLC.
- Author:
Fei LIU
1
;
Yang YANG
;
Xiaojie TAN
;
Kaishun BI
;
Ying JIA
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; Chromatography, Reverse-Phase; Chrysanthemum; chemistry; Drugs, Chinese Herbal; analysis; Flavonoids; analysis
- From: China Journal of Chinese Materia Medica 2009;34(16):2067-2070
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop an HPLC method for the determination of luteolin-7-O-glycoside, apigenin-7-O-glycoside, linarin, luteolin, apigenin and acacetin in Flos Chrysanthemi Indici simultaneously.
METHODThe reversed phase HPLC system consisting of a C18 column (4.6 mm x 250 mm, 5 microm) and a mixture of acetonitrile and 0.05% phosphate acid with gradient elution as the mobile phase was adopted. The absorbance was monitored at 326 nm.
RESULTThe linear response range was 1.08-21.5 mg x L(-1) (r = 0.9990), 0.278-5.57 mg x L(-1) (r = 0.9994), 5.58-112 mg x L(-1) (r = 0.9999), 0.521-10.4 mg x L(-1) (r = 0.9995), 0.162-3.25 mg x L(-1) (r = 0.9999) and 0.288-5.76 mg x L(-1) (r = 0.9998), respectively (n = 6). The average recoveries (n = 9) of six flavonoids were 96.34%-104.0%. All of RSD of precision (n = 6) and repeatability (n = 6) were less than 1.2% and 2.6%.
CONCLUSIONThe validation data demonstrated that the method was accurate and repeatable, and can be ase to measure the six flavonoids in Flos Chrysanthemi Indici.
