Determination of militarine in Rhizoma Bletillae by HPLC.
- Author:
Xun HE
1
;
Aimin WANG
;
Yongjun LI
;
Yonglin WANG
;
Shuyong ZHOU
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; methods; Drugs, Chinese Herbal; analysis; Orchidaceae; chemistry; Succinates; analysis
- From: China Journal of Chinese Materia Medica 2009;34(16):2076-2078
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop an HPLC method for determination of militarine in Rhizoma Bletillae.
METHODAnalysis was carried out on a Symmetry C18 analytical column, and a C18 guard column eluted with mobile phase consisted of acetonitrile and water (37:63) militarine the detection wavelength was set at 244 nm.
RESULTThe contents of in 12 samples of Rhizoma Bletillae in market were in the ranges of 1.45%-3.32%.
CONCLUSIONThe method is simple, accurate and reproducible, and is suitable for the quality control of Rhizoma Bletillae.
