Chemical constituents and bioactivity of Teucrium pilosum.
- Author:
Mingyue MOU
1
;
Qianjun ZHANG
;
Wenyi KANG
;
Ke PI
;
Qing CHEN
;
Rongjun YAO
Author Information
- Publication Type:Journal Article
- MeSH: Antifungal Agents; chemistry; isolation & purification; Antioxidants; chemistry; Enzyme Inhibitors; chemistry; isolation & purification; Fusarium; drug effects; Glycoside Hydrolase Inhibitors; Plant Extracts; chemistry; isolation & purification; Teucrium; chemistry; alpha-Glucosidases; analysis
- From: China Journal of Chinese Materia Medica 2009;34(17):2189-2193
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the chemical constituents and bioactivity of Teurium pilosum.
METHODVarious column chromatographic techniques were used to isolate the constituents. A combination of EI-MS, NMR spectroscopy and X-Ray were applied to identify the structures. The anti-microorganism was accomplished by disk diffusion method, the antioxidant activity was assayed by the DPPH microanalysis models and the inhibitory activity of alpha-glucosidase was screened In vitro.
RESULTEight compounds were isolated and identified as: glyceryl tristearate (1), 2,5-dioxolanone (2), fernenol (3), stigmasta-5,22-dien-3P-ol (4), 24-nor cholesta-5,22 (E)-dien-3beta-ol (5), ca-spinasterol (6), (3,4-dihydroxyphenyl) acrylate (7), 3,4-dihydroxy phenyl acrylic acid (8).
CONCLUSIONAll compounds have been isolated from the genus for the first time. Compound 3 [IC50 = (37.63 +/- 3.45) mg +/- L(-1)], 6 [IC50 = (178.92 +/- 4.99) mg x L(-1)] and 8 [IC50 = (44.32 +/- 7.02) mg x L(-1)] are of higher inhibitory alpha-glucosidase activity than that of acarbose [IC50 = (1081.27 +/- 12.3) mg x L(-1)]. Compound7 [IC50 = (4.81 +/- 0.96) mg x L(-1)] and 8 [IC50 = (4.16 +/- 0.11) mg L(-1)] showed higher antioxidant activity than that of BHT [IC50 = (35.64 +/- 0.36) mg x L(-1)] and BHA [IC50 = (8.74 +/- 0.39) mg x L(-1)]. Compound 5-8 exhibited inhibitory activity against Fusarium graminearum. Compound 5 and 8 showed inhibitory activity against Botrytis cinerea.