Transport mechanism of swertiamain metabolite across Caco-2 model.
- Author:
Cheng TANG
1
;
Yanjun ZHANG
;
Tiejun ZHANG
;
Chengwang TIAN
;
Changxiao LIU
Author Information
- Publication Type:Journal Article
- MeSH: Biological Transport; Caco-2 Cells; Cell Membrane Permeability; Drugs, Chinese Herbal; chemistry; pharmacokinetics; Humans; Models, Biological
- From: China Journal of Chinese Materia Medica 2009;34(17):2258-2260
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the intestinal absorption mechanism of swertiamain metabolite {(Z)-5-ethylidene-8-hydroxy-3,4,5,6,7,8-hexahydro-1H-pyrano [3,4-c]pyridine-1-one, EHPO}.
METHODThe time depended hi-directional transport of EHPO in Caco-2 monolayer model was investigated with the factors of concentration, pH and verapamil. EHPO concentration was measured by HPLC assay and the apparent permeability coefficients (Papp) were calculated.
RESULTIn the this cell model, EHPO could be absorbed through Caco-2 soon. The P(app AP-BL) was equal to the P(app BL-AP), and Papp keep almost constant with the selected concentration investigated. But the Papp could be influenced by pH and verapamil (100 mg x L(-1)).
CONCLUSIONThe absorption of EHPO in Caco-2 cell model is a passive one. And absorption of EHPO in the intestines is quite good.
